Coding

Part:BBa_K4140021:Design

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-28)


AcrIIA5 v2 anti-CRISPR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
    Illegal EcoRI site found at 521
    Illegal XbaI site found at 25
    Illegal XbaI site found at 530
    Illegal SpeI site found at 19
    Illegal SpeI site found at 539
    Illegal PstI site found at 1
    Illegal PstI site found at 565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
    Illegal EcoRI site found at 521
    Illegal SpeI site found at 19
    Illegal SpeI site found at 539
    Illegal PstI site found at 1
    Illegal PstI site found at 565
    Illegal NotI site found at 43
    Illegal NotI site found at 502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
    Illegal EcoRI site found at 521
    Illegal BglII site found at 13
    Illegal BglII site found at 548
    Illegal BamHI site found at 37
    Illegal BamHI site found at 513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
    Illegal EcoRI site found at 521
    Illegal XbaI site found at 25
    Illegal XbaI site found at 530
    Illegal SpeI site found at 19
    Illegal SpeI site found at 539
    Illegal PstI site found at 1
    Illegal PstI site found at 565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
    Illegal EcoRI site found at 521
    Illegal XbaI site found at 25
    Illegal XbaI site found at 530
    Illegal SpeI site found at 19
    Illegal SpeI site found at 539
    Illegal PstI site found at 1
    Illegal PstI site found at 565
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design considerations


Source

By team members

References